Albani's father was a silk merchant who intended to instruct his son in the same trade; but by age twelve, Albani became an apprentice under the competent mannerist painter Denis Calvaert, where he met Guido Reni. Soon he followed Reni to the so-called "Academy" run by the Carracci family: Annibale, Agostino, and Ludovico. This studio fostered the careers of many painters of the Bolognese school, including Domenichino, Massari, Viola, Lanfranco, Giovanni Francesco Grimaldi, Pietro Faccini, Remigio Cantagallina, and Reni.

In the year 1600, Albani moved to Rome to work on the fresco decoration of the gallery of the Palazzo Farnese being completed by the studio of Annibale Carracci under Annibale's direction. Rome, under Clement VIII Aldobrandini(1592-1605) was exhibiting some degree of administrative stability and renewed artistic patronage. While Pope Clement was born from a Florentine family residing in Urbino, his family was allied by marriage to the Emilia-Romagna and the Farnese, since Ranuccio I Farnese, Duke of Parma had married Margherita Aldobrandini. Parma, like Bologna, are part of the Region of Emilia-Romagna. Thus it was no surprise that Cardinal Odoarde Farnese, Ranuccio's brother, chose the Carraccis from Bologna for patronage, thereby establishing Bolognese predominance of Roman fresco painting for nearly two decades. Albani became one of Annibale's most prominent apprentices. Using Annibale's designs and assisted by Lanfranco and Sisto Badalocchio, Albani completed frescoes for the San Diego Chapel in San Giacomo degli Spagnoli between 1602-1607. In 1606-7, Albani completed the frescoes in the Palazzo Mattei di Giove in Rome. He later completed two other frescoes in the same palace, also on the theme of Life of Joseph.

In 1609, he completed the ceiling of a large hall with Fall of Phaeton and Council of the Gods for the Palazzo Giustianini (now Odescalchi) at Bassano (di Sutri) Romano. This work was commissioned by the Marchese Vicenzo Giustiniani, famous for also being patron to Caravaggio. During 1612-14, Albani completed the Choir frescoes at the newly remodeled (by Pietro da Cortona) church of Santa Maria della Pace. In 1616 he painted ceiling frescoes of Apollo and the Seasons at Palazzo Verospi in Via del Corso for the cardinal Fabrizio Verospi.

In 1617 he returned to Bologna and produced altarpieces, allegorical paintings, and idyllic landscapes. In later years, Albani developed a mutual, though respectful, rivalry with the more successful Guido Reni, who was also heavily patronized by the Aldobrandini, and under whom Albani had worked under at the chapel of the Palazzo del Quirinale. Albani never acquired the monumentality or tenebrism that was quaking the contemporary world of painters, and in fact, he is derided often for his lyric, cherubim-filled sweetness, which often has not yet shaken the mannerist elegance. While Albani's thematic would have appealed to Poussin, he lacked the Frenchman's muscular drama. Yet his paintings on mythological and poetic subjects have a sweetness and charm that makes his success completely understandable.

Selected Biblography: The standard study in English is Catherine Puglisi, Francesco Albani (New Haven: Yale University Press, 1999). Also of interest: Elena Rossoni, Francesco Albani a San Giovanni in Persiceto (Bologna: Minerva Edizioni, 2005 and sections in the standard histories of 17th-century Italian art.